In this article we will see how we can get the border of the label in the labelled image within the given region in mahotas. For this we are going to use the fluorescent microscopy image from a nuclear segmentation benchmark. We can get the image with the help of command given below
mahotas.demos.nuclear_image()
Below is the nuclear_image
In order to do this we will use mahotas.labelled.border method
Syntax : mahotas.labelled.border(labelled_image, i, j)
Argument : It takes numpy.ndarray object i.e labelled image and two integer as argument
Note : A pixel is on the border if it has value i (or j)
Return : It returns numpy.ndarray object i.e labelled image with border label
Note : The input of the this should be the filtered image object which is labelled
In order to filter the image we will take the image object which is numpy.ndarray and filter it with the help of indexing, below is the command to do this
image = image[:, :, 0]
Example 1 :
Python3
# importing required libraries import mahotas import numpy as np from pylab import imshow, show import os # loading nuclear image f = mahotas.demos.load( 'nuclear' ) # setting filter to the image f = f[:, :, 0 ] # setting gaussian filter f = mahotas.gaussian_filter(f, 4 ) # setting threshold value f = (f> f.mean()) # creating a labelled image labelled, n_nucleus = mahotas.label(f) # showing the labelled image print ( "Labelled Image" ) imshow(labelled) show() # getting border for label at given point relabeled = mahotas.labelled.border(labelled, 0 , 20 ) # showing the image print ( "Labels With borders at given point" ) imshow(relabelled) show() |
Output :
Example 2 :
Python3
# importing required libraries import numpy as np import mahotas from pylab import imshow, show # loading image img = mahotas.imread( 'dog_image.png' ) # filtering the image img = img[:, :, 0 ] # setting gaussian filter gaussian = mahotas.gaussian_filter(img, 15 ) # setting threshold value gaussian = (gaussian > gaussian.mean()) # creating a labelled image labelled, n_nucleus = mahotas.label(gaussian) print ( "Labelled Image" ) # showing the gaussian filter imshow(labelled) show() # getting border for label at given point relabeled = mahotas.labelled.border(labelled, 1 , 0 ) # showing the image print ( "Labels With borders at given point" ) imshow(relabelled) show() |
Output :